9.4 Multiplexed Proteomics for Detection of Specific Proteins in Gels and on Blots

Molecular Probes is the leader in the developing area of Multiplexed Proteomics — the simultaneous detection of multiple protein targets in a single sample. Our detection methodology typically involves differential staining of the entire protein profile and specific proteins, such as glycoproteins or epitope-tagged proteins, in two or more visually distinguishable colors. Our Pro-Q, DyeChrome and Amplex Gold kits and reagents, which are described in this section, use novel specific protein detection reagents in combination with some of the general protein stains described in Section 9.3 for dichromatic — or even trichromatic — staining of proteins on the same gel or blot. Multiplex detection reduces the number of duplicate gels that must be run and ensures the spatial registration of protein spots in the sample.